A Fanca knockout mouse model reveals novel Fancd2 function
WEBFeb 12, 2024 · We found that both the Fanca KO and Fancd2 KO cause severe FA phenotypes in mice. However, Fanca KO mice exhibited milder FA phenotypes comparing to Fancd2 KO mice. Fanca KO mice showed higher embryonic and postnatal survival rate, less congenital eye defects in early development.
Fancd2 in vivo interaction network reveals a non-canonical role …
WEBApr 5, 2017 · Here we developed a Flag- and hemagglutinin-tagged Fancd2 knock-in mouse strain that allowed a high throughput mass spectrometry approach to search for Fancd2-binding proteins in...
Fanconi anemia proteins participate in a break-induced ... - Nature
WEBJun 10, 2021 · To examine this possibility, we obtained FancL knockout-first (FancL kf/kf) mice, which exhibited FancL messenger RNA levels 5–10% of those of wild-type mice (Extended Data Fig. 8a–d).
Fanci knockout mouse model reveals common and distinct …
WEBJun 20, 2019 · Mice deficient in both aldehyde dehydrogenase 2 and FANCD2 (Aldh2 −/− Fancd2 −/−) exhibited a more severe FA phenotype, including aplastic anemia, leukemia and congenital defects when compared to Fancd2 −/− mice .
FANCD2 maintains replication fork stability during ... - Nature
WEBMay 27, 2022 · FANCD2 maintains replication fork stability during misincorporation of the DNA demethylation products 5-hydroxymethyl-2’-deoxycytidine and 5-hydroxymethyl-2’-deoxyuridine. María José...
A Fanci knockout mouse model reveals common and distinct ... - PubMed
WEBAug 22, 2019 · A critical step in the activation of FA pathway is the monoubiquitination of FANCD2 and its binding partner FANCI. To better address the clinical phenotype associated with FANCI and the epistatic relationship with FANCD2, we created the first conditional inactivation model for FANCI in mouse.
Histone H2AX and Fanconi anemia FANCD2 function in the same …
WEBFeb 15, 2007 · The central FA protein FANCD2 is known to relocate to chromatin upon DNA damage in a poorly understood process. Here, we have induced subnuclear accumulation of DNA damage to prove that histone H2AX is a novel component of the FA/BRCA pathway in response to stalled replication forks.
Severe Fanconi Anemia phenotypes in Fancd2 depletion mice
WEBJun 30, 2019 · FANCD2 protein play the central role in FA pathway. To study the in vivo role of FANCD2, we generated and characterized a new Fancd2 knockout mouse strain with 7bp deletion in Fancd2 gene 5' terminus using Crispr-Cas9 in …
A Fanca knockout mouse model reveals novel Fancd2 function
WEBFeb 12, 2024 · We found that both the Fanca KO and Fancd2 KO cause severe FA phenotypes in mice. However, Fanca KO mice exhibited milder FA phenotypes comparing to Fancd2 KO mice. Fanca KO mice showed higher embryonic and postnatal survival rate, less congenital eye defects in early development.
Fancd2 is required for the repression of germline transposable …
WEBTo investigate the mechanistic link between the Fancd2/FA pathway and Prmt5-directed TE silencing in early PGCs, we generated three mouse models: 1) Fancd2-KO;Pou5f1-eGFP for the effect of Fancd2 deficiency on Prmt5-mediated H2A/H4R3me2s repressive chromatin modifications on LINE1 TEs; 2) Fanca-KO;Pou5f1-eGFP for the requirement of …