GST-tagged Proteins – Production and Purification
WEBThe GST tag. Protein purification with affinity tags such as glutathione S-transferase (GST), histidine (HIS), and other affinity tags, enables purification of proteins with both known and unknown biochemical properties.
Glutathione S-transferase - Wikipedia
WEBGlutathione S-transferases (GSTs), previously known as ligandins, are a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification.
Purification of proteins fused to glutathione S-tranferase
WEBThis chapter describes the use of glutathione S-transferase (GST) gene fusion proteins as a method for inducible, high-level protein expression and purification from bacterial cell lysates. The protein is expressed in a pGEX vector, with the GST moiety located at the N-terminus followed by the target protein.
A Glutathione-S-transferase Fusion Protein Nanosensor
WEBOct 10, 2020 · Quantifying fusion-tagged proteins normally requires several purification steps coupled with classical protein assays. Here, we developed a broadly-applicable nanosensor platform that quantifies glutathione-S-transferase (GST) fusion proteins in …
Overview of Affinity Tags for Protein Purification
WEBGST fusion proteins can be detected by a colorimetric assay with the GST substrate 1-chloro-2,4-dinitrobenzene (CDNB) or with anti-GST antibodies (e.g., GE Healthcare, Sigma, BD Biosciences). Precipitations of GST fusion proteins with glutathione-coupled beads are commonly used for the detection of protein-protein or protein-DNA interactions.
Transglutaminase 2 crosslinks the glutathione S-transferase tag ...
WEBJan 13, 2021 · These results indicated that TG2 interferes with the protein–protein interactions of GST-fused proteins by crosslinking the GST tag; therefore, a GST4QN tag could improve the reproducibility...
GST-tagged protein purification overview - Takara Bio
WEBGST-tagged protein purification overview. Glutathione-Superflow Resin allows rapid affinity purification of GST-tagged proteins for batch, gravity flow, and FPLC applications. The resin is based on 6% cross-linked agarose, with glutathione covalently bound to the resin.
Purification of GST-Tagged Proteins - PubMed
WEBThis protocol describes the purification of recombinant proteins fused to glutathione S-transferase (GST, GST-tagged proteins) by Glutathione Affinity purification. The GST tag frequently increases the solubility of the fused protein of interest and thus enables its purification and subsequent functional characterization.
Purification of GST-Tagged Proteins - ScienceDirect
WEBJan 1, 2015 · This protocol describes the purification of recombinant proteins fused to glutathione S-transferase (GST, GST-tagged proteins) by Glutathione Affinity purification.
Purification of GST-Tagged Proteins - ScienceDirect
WEBJan 1, 2015 · Glutathione Affinity is an efficient method for single-step purification of proteins fused to a GST (glutathione S-transferase) tag.
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